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KMID : 0984920140160020108
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Journal of Skin Barrier Research
2014 Volume.16 No. 2 p.108 ~ p.108
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Inhibition of the Extrinsic Aging-related Ion Channels TRPV1 and ORAI1 by Constituents of the Fruits of Foeniculum Vulgare
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Nam Joo-Hyun
Kim Woo-Kyung
Lee Dong-Ung
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Abstract
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Ultraviolet (UV) exposure due to the solar radiation is the most important cause of extrinsic skin aging (photoaging), which is characterized clinically by deep skin wrinkling and pigmentation. These phenomena are due to the increasing of metalloproteinases-1 (MMP-1) expression in keratinocytes and tyrosinase activation in melanocytes. In a recent study, it was reported that two Ca2+ channels, TRPV1 and ORAI1, are involved in UV-induced MMP-1 expression and tyrosinase activity, respectively. In the present study, we evaluated whether the fruits of Foeniculum vulgare (fennel) have inhibitory effects on TRPV1 and ORAI1 using the whole-cell patch-clamp technique and intracellular Ca2+ measurements. n our electrophysiological study of the extract and its fractions, the dichloromethane and hexane fractions were found to strongly block capsaicin-induced TRPV1 and ORAI1 currents. Furthermore, of the 18 compounds isolated from the hexane and dichloromethane fractions, trans-anethole in the hexane fraction had inhibitory effects on both ORAI1 and increases in cytoplasmic Ca2+ concentrations in response to ORAI1 activation (both by ~70 % at 100 μM). Our findings suggest that the fruit extract of F. vulgare and provide possible novel ways of treating and preventing UV-induced skin aging.
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KEYWORD
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Foeniculum vulgare' Extrinsic aging, TRPV1, ORAI-1, Ultraviolet
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